Abbkine CheKine™ NADP/NADPH Quantification/Ratio Determination Assay Kit contains below components:
NADPH Extraction BufferNADP Extraction BufferNADPH standard (10 mM)NADP Cycling Enzyme MixPES SolutionMTT SolutionGlucose (1M)Assay Buffer
The Abbkine CheKine™ NADP/NADPH Cell Metabolism Assay Kit provides a convenient tool for sensitive detection of the NADP and NADPH, and their ratio in various tissues and subcellular organelles. This Kit is based on enzyme cycling reaction (It does not recognize NAD+/NADH), in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at OD565 nm, is proportionate to the NADP+ /NADPH concentration in the sample.
Our NADP/NADPH Quantification Kit has below features:
1. Detect NADP+/NADPH concentrations and ratios in cell or tissue extracts.
2. Detection limit of 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.
About Abbkine Scientific Co., Ltd.
Abbkine focuses on Proteomics and Cytology. we not only supply products in proteomics research, from basic Immunology products such as Protein Extraction and Quantification kits to Tag Antibodies, Primary Antibodies and Secondary Antibodies, but also offer products in cytology research, from dyes and kits for cell status detection, organelle extraction kits, cell substructure staining and cell metabolism detection products, to cytokine for cell culture and protein detection kits, to help your research career.
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